Evidence based sperm DNA fragmentation
نویسنده
چکیده
منابع مشابه
Studying of the Expression of BAX and BCL-2 Genes in Men with Sperm DNA Fragmentation Based on DFI and Comet Assay
Introduction: The amount of expression of BAX and BCL-2 genes in infertile men’s sperm as well as its association with sperm parameters and DNA fragmentation index is an issue which has not been studied yet. In this research, it is assumed that up-regulation of BAX and downregulation of BCL-2 are directly associated with sperm DNA fragmentation. Methods: After obtaining semen samples from the ...
متن کاملP-13: Comparison of Sperm Quality, Oxidative Stress, DNA Fragmentation, Protamine Deficiency, and DNA Methylation in Varicocele and Fertile Individuals
s:1993:"Background: There are many approaches that gene expression is controlled in eukaryotes. DNA methylation is one of several epigenetic mechanisms that cells use to control gene expression and lock genes in the "off" position. In addition, sperm DNA damage can correlate with DNA methylation defect. There is evidence that sperm of infertile men contain more DNA damage than fertile men and t...
متن کاملO-15: Different Leukocyte Concentrations in Normal and Abnormal Human Semen and Its Correlation with Sperm Intracellular Reactive Oxygen Species, Lipid Peroxidation and DNA Fragmentation
Background: According to World Health Organization guideline (WHO 2010), seminal leukocyte concentration over 1×106/mL is a threat for sperm quality because of their role in reactive oxygen species (ROS) production, lipid per-oxidation (LPO) and later DNA fragmentation (DF). However, this threshold is equal for all semen samples without attention to their qualities. Therefore this study was set...
متن کاملEffects of Sperm DNA Fragmentation on Semen Parameters and ICSI Outcome Determined by an Improved SCD Test, Halosperm
Background Sperm DNA fragmentation is known as an important cause of male infertility. The influence of sperm DNA damage on reproductive potential has been subject of many studies indicating various results and remaining the subject controversial. In this study, we investigated differences of the semen parameters and intracytoplasmic sperm injection (ICSI) outcome according to sperm DNA fragmen...
متن کاملP-19: Effect of Three Sperm Preparation Methods on Sperm DNA Integrity and Oocyte Fertilization Rate
Background: Techniques of sperm preparation require centrifugation to separate spermatozoa from the seminal plasma. The centrifugation can induce damage to the spermatozoa and increase reactive oxygen species(ROS) productionspecially in leukospermic speciemens that are associated with impaired function and fertilizing capacity. However in ICSI, fertilization can occure with damage DNA sperm but...
متن کاملP-81: Evaluation of DNA Fragmentation in Patients with Total Globozoospermia
Background: Infertility is estimated to affect up to 15% of couples of reproductive age, and male factors contribute to 50% of these cases. Globozoospermia syndrome is a severe form of teratozoospermia, with an incidence of less than 0.1%. It is characterized by round sperm head. The most prominent feature of globozoospermia is the malformation of the acrosome, which is totally absent in the to...
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